Laboratory of Analytical Chemistry for Pharmaceutical Sciences
学術論文（2023年度）
1. Yamamoto S, Kato N, Wada M, Kinoshita N, A rapid and convenient enzyme digestion method for the analysis of N-glycans using exoglycosidase-impregnated polyacrylamide gels fabricated in an automatic pipette tip, Anal. Sci., 39 (2023) 1041-1046 DOI: https://doi.org/10.1007/s44211-023-00314-8
学術論文（2022年度）
1. Yamamoto S, Miyawakii N, Kawakami N, Kinoshita N, Suzuki S, Study of
HPLC Separation and Fractionation of 8-aminopyrene-1, 3, 6-trisulfonic
Acid Labeled N-glycans Using a Hydrophilic Interaction Chromatography Column,
BUNSEKIKAGAKU, 71 (2022) 333-339 DOI: https://doi.org/10.2116/bunsekikagaku.71.333
2. Morikawa Y, Nishiwaki K, Kinoshita M, Suzuki S, Nakanishi I, Development
of a cost-effective laser diode-induced fluorescence detection instrument
for cyanide detection , Anal. Sci., 38 (2022) 437-442 DOI:https://doi.org/10.1007/s44211-022-00065-y
学術論文（2021年度）
1. Yamamoto S, Yano S, Kinoshita M, Suzuki S, In Situ Pinpoint Photopolymerization of Phos-Tag Polyacrylamide Gel in
Poly(dimethylsiloxane)/Glass Microchip for Specific Entrapment, Derivatization,
and Separation of Phosphorylated Compounds, Gels.,7 (2021) 268 DOI: https://doi.org/10.3390/gels7040268
2. Kinoshita M, Yamada K, Recent advances and trends in sample preparation and chemical modification
for glycan analysis, J. Pharm.Biomed. Anal., 207 (2022) 114424 DOI: https://doi.org/10.1016/j.jpba.2021.114424
3. Kinoshita M, Nakajima K, Yamamoto S, Suzuki S, High-throughput N-glycan screening method for therapeutic antibodies
using a microchip-based DNA analyzer: a promising methodology for monitoring
monoclonal antibody N-glycosylation, Anal. Bioanal. Chem., 413 (2021) 4727-4735
DOI: https://doi.org/10.1007/s00216-021-03434-0
4. Yamamoto S, Maetani K, Tatsumi G, Okada F, Kinoshita M, Suzuki S, Nylon Monofilament Mold Three-dimensional Microfluidic Chips
for Size-exclusion Microchip Electrophoresis: Application to Specific Online
Preconcentration of Proteins, Anal. Sci., 37 (2021) 1511-1516 DOI: https://doi.org/10.2116/analsci.21P080
5. Hioki M, Kobayashi H, Kinoshita M, Yamamoto S, Suzuki S, Chromatographic Performance of an Amine/amino-bonded Column
and a Monolithic Reversed-Phase Column for the Separation of Fluorescently
Labeled Glycoprotein Glycans, Chromatography, 42 (2021) 99-107 DOI: https://doi.org/10.15583/jpchrom.2021.005.
6. Kinoshita M, Nakayani Y, Yamada K, Yamamoto S, Suzuki S, A rapid and facile preparation of APTS-labeled N-glycans by combination of ion pair-assisted extraction and HILIC-SPE for routine glycan analysis, J. Pharm.Biomed. Anal. 195 (2021) 113875 DOI: https://doi.org/10.1016/j.jpba.2020.113875
1. Yamamoto S, Ueda M, Kasai M, Ueda Y, Kinoshita M, Suzuki S, A fast and convenient solid phase preparation method for releasing N-glycans from glycoproteins using trypsin- andpeptide-N-glycosidase F (PNGase F)-impregnated polyacrylamide gels fabricated in a pipette tip, J, Pharm. Biomed. Anal. (2020) 112995.
2. Kinoshita M, Saito A, Yamamoto S, Suzuki S, A practical method for preparing fluorescent-labeled glycans with a 9-fluorenylmethyl derivative to simplify a fluorimetric HPLC-based analysis, J, Pharm. Biomed. Anal. (2020) 113267.
3. Kishimoto Y, Okada F, Maesako T, Yamamoto S, Kinoshita M, Hayakawa T, Suzuki S,Analysis of 2-aminopyridine labele d glycans by dual-mode online solid phase extraction for hydrophilic interaction and reversed-phase liquid chromatography, J. Chromatogr. A (2020) 461194.
4. Kinoshita M, Yamamoto S, Suzuki S, Age-Related Changes in O‑Acetylation of Sialic Acids Bound to N‑Glycans of Male Rat Serum Glycoproteins and Influence of Dietary Intake on Their Changes, ACS Omega (2020) 18608-18618
学術論文（2019年度）
1. Yamamoto S, Fujiwara H, Maruyama K, Tanaka Y, Kinoshita M, Suzuki S, Simultaneous determination of inorganic anions and cations in water and biological samples by capillary electrophoresis with a capacitive coupled contactless conductivity detector using capillary filling method, Anal. Sci.(2019) 295-300.
2. Yamamoto S, Hayashi Y, Matsunaga H,Okada F, Kinoshita M, Suzuki S, Analysis of 2-Aminobenzoic Acid-Labeled Monosaccharides and Glycoprotein-Derived Oligosaccharides by Online Cleanup Liquid Chromatography in the Reversed-Phase and Hydrophilic Interaction Liquid Chromatography Modes, Chromatography (2019) 65-70
3. Utamura N, Yamamoto S, Kinoshita M, Suzuki S, A new separation mode for non-aqueous capillary electrophoresis using excess adsorption of cationic electrolytes, BUNSEKI KAGAKU (2019) 859-864.
学術論文（2018年度）
1. Yamamoto S, Okada F, Kinoshita M, Suzuki S,On-line microchip electrophoresis-mediated preconcentration of cationic compounds utilizing cationic polyacrylamide gels fabricated by in situ photopolymerization, Analyst (2018) 4429-4435.
2. Yamada K, Kayahara H, Kinoshita M, Suzuki S,Simultaneous Analysis of Sulfated and Phosphorylated Glycans by Serotonin-Immobilized Column Enrichment and Hydrophilic Interaction Chromatography, Anal. Chem. (2018) 8387-8395
3. Yamamoto S, Nishida N, Kinoshita M, Suzuki S,On-line derivatization and concentration of aspartic acid using in situ photopolymerized carboxylic acid type polyacrylamide gels as a permselective preconcentrator, Chromatography (2018) 125-130
4. Yamamoto S, Kinoshita M, Ikegami T, Suzuki S, High-performance liquid chromatographic separation of 8-aminopyrene-1,3,6-trisulfonic acid labeled N-glycans using a functional tetrazole hydrophilic interaction liquid chromatography column, J. Chromatogr. A (2018) 44-162
学術論文（2017年度）
1. Yamagami M, Matsui Y, Hayakawa T, Yamamoto S, Kinoshita M Suzuki S,
Plug-plug kinetic capillary electrophoresis for in-capillaryexoglycosidase
digestion as a profiling tool for the analysis ofglycoprotein glycans Oligosaccharides,
J. Chromatogr. A (2017) 157-162.
2. Yamada Y, Matsumura C, Yamada K, Teshima K, Hiroshima T, Kinoshita M,
Suzuki S, Kakehi K, Combination of SDS-PAGE and intact mass analysis for
rapid determination of heterogeneities in monoclonal antibody therapeutics,
Electrophoresis (2017) 1344-1352
3. Nagatomo Y, Hashimoto S, Kishimoto Y, Hayakawa T, Yamamoto S, Kinoshita M Suzuki S Online Cleanup Liquid Chromatography for the Analysis of Glycoprotein-Derived
Oligosaccharides Labeled with 7-Amino-4-methylcoumarin, Chromatography
(2017) 23-30
4. Yamamoto S, Himeno M, Kobayashi M, Akamatsu M, Satoh R, Kinoshita M, Sugiura
R, Suzuki S Microchip electrophoresis utilizing an in situ photopolymerized Phos-tag
binding polyacrylamide gel for specific entrapment and analysis of phosphorylated
compounds, Analyst (2017) 3416-3423
学術論文（2016年度）
1. Takeda Y, Hayashi Y, Utamura N, Takamoto C, Kinoshita M, Yamamoto S, Hayakawa
T, Suzuki S, Capillary Electrochromatography Using Monoamine- and Triamine-bonded
Silica Nanoparticles as Pseudostationary Phases, J. Chromatogr. A (2016) 170-176
2. Yamamoto S, Kinoshita M, Suzuki S, Current landscape of protein glycosylation
analysis and recentprogress toward a novel paradigm of glycoscience research,
J, Pharm. Biomed. Anal. (2016) 273-300
学術論文（2015年度）
1. Yamamoto S, Nagai E, Asada Y, Kinoshita M Suzuki S., A Rapid and Highly
Sensitive Microchip Electrophoresis of Mono- and Mucin-type Oligosaccharides
Labeled with 7-Amino-4-methylcoumarin. Anal. Bioanal. Chem. (2015) 1499-1503
2. Yamamoto S, Tamata Y, Sejima K, Kinoshita M Suzuki S., Chiral Separation
of D/L-Aldoses by Micellar Electrokinetic Chromatography Using a Chiral
Derivatization Reagent and a Phenylboronic Acid Complex. Anal. Bioanal.
Chem. (2015) 6201-6206
3. Yamamoto S, Iwata T, Nishiwaki K, Kinoshita M Suzuki S, Synthesis of Quaternary Ammonium Derivatives of Cellulose as the Coating
Reagents for Capillary Electrophoresis. Chromatography (2015) 93-98
学術論文（2014年度）
1. Iwatsuka K, Iwamoto H, Kinoshita M, Inada K, Yasueda S, Kakehi K., Comparative
Studies of N-Glycans and Glycosaminoglycans Present in SIRC (Statens Seruminstitut
Rabbit Cornea) Cells and Corneal Epithelial Cells from Rabbit Eyes. Curr
Eye Res. (2014) 686-694
2. Kinoshita M, Mitsui Y, Kakoi N, Yamada K, Hayakawa T, Kakehi K., Common
glycoproteins expressing polylactosamine-type glycans on matched patient
primary and metastatic melanoma cells show different glycan profiles, J
Proteome Res. 13(2) (2014) 1021-1033
3. Suzuki S., Highly Sensitive Methods Using Liquid Chromatography and
Capillary Electrophoresis for Quantitative Analysis of Glycoprotein Glycans
different glycan profiles, Chromatography 35 (2014) 1-22
4. Yagi Y, Kakehi K, Hayakawa T, Suzuki S., Application of Microchip Electrophoresis
Sodium Dodecyl Sulfate for the Evaluation of Change of Degradation Species
of Therapeutic Antibodies in Stability Testing, Anal. Sci. 30 (2014) 483-488 DOI:http://dx.doi.org/10.2116/analsci.30.483
6. Higashi K, Asano K, Yagi M, Yamada K, Arakawa T, Ehashi T, Mori T, Sumida K, Kushida M, Ando S, Kinoshita M, Kakehi K, Tachibana T, saito K, Expression of the Clustered NeuAca2-3Galβ O-glycan Determines the Cell Differentitation State of the Cell, J. Biol. Chem. 289 (2014) 25833-25843